| Annotated protein: | SLIT and NTRK-like protein 5. Gene symbol: SLITRK5. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q810B7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLITRK5 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of trans-synaptic signaling by BDNF, modulating synaptic transmission (GO:0150035) |
| Annotated paper: | Song M, et al. "Slitrk5 Mediates BDNF-Dependent TrkB Receptor Trafficking and Signaling" Dev Cell. 2015 Jun 22;33(6):690-702 PMID:26004511 |
| Figure(s): | Figure 1, 4, 5, 6, 7 |
| Annotation description: | Figure 1: TrkB receptors interact and co-localize with Slitrk5 in HEK293T cells. Super resolution imaging of DIV6 striatal neurons, treated with or without BDNF, showed surface-TrkB-receptors colocalized with Slitrk5 in a BDNF-dependent manner. Figure 2: PTPδ and TrkB compete for binding to Slitrk5. Figure 5: Slitrk5 facilitates TrkB receptor recruitment into Rab11-positive compartments. Figure 6: Slitrk5 interacts with Rab11-FIP3 to facilitate TrkB receptor trafficking to Rab11-positive recycling endosomes Authors conclude; "Here, we have shown that Slitrk5, a postsynaptic plasma membrane protein containing extracellular LRR domains, interacts under basal conditions with the presynaptic adhesion molecule PTPδ in trans, but in the presence of BDNF, shifts to a cis-interaction with TrkB receptor that mediates its postendocytic recycling, leading to functional resensitization of neurotrophic signaling." "Here, we report that Slitrk5 modulates BDNF-dependent biological responses through direct interaction with TrkB receptors. Under basal conditions, Slitrk5 interacts primarily with a trans-synaptic binding partner, PTPδ; however, upon BDNF stimulation, Slitrk5 shifts to cis-interactions with TrkB. In the absence of Slitrk5, TrkB has a reduced rate of ligand-dependent recycling and altered responsiveness to BDNF treatment" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4805 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |