| Annotated protein: | SLIT and NTRK-like protein 5. Gene symbol: SLITRK5. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q810B7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLITRK5 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Annotated paper: | Song M, et al. "Slitrk5 Mediates BDNF-Dependent TrkB Receptor Trafficking and Signaling" Dev Cell. 2015 Jun 22;33(6):690-702 PMID:26004511 |
| Figure(s): | Figure 1, 4, 5 |
| Annotation description: | Figure 1: TrkB receptors interact and co-localize with Slitrk5 in HEK293T cells. Super resolution imaging of DIV6 striatal neurons, treated with or without BDNF, showed surface-TrkB-receptors colocalized with Slitrk5 in a BDNF-dependent manner. Figure 2: PTPδ and TrkB compete for binding to Slitrk5. Figure 5: Slitrk5 facilitates TrkB receptor recruitment into Rab11-positive compartments. Figure 6: Slitrk5 interacts with Rab11-FIP3 to facilitate TrkB receptor trafficking to Rab11-positive recycling endosomes While co-localization with a PSD marker is not shown, authors inferred from these observations that Slitrk5 is localized to PSD membrane; "Here, we have shown that Slitrk5, a postsynaptic plasma membrane protein containing extracellular LRR domains, interacts under basal conditions with the presynaptic adhesion molecule PTPδ in trans, but in the presence of BDNF, shifts to a cis-interaction with TrkB receptor that mediates its postendocytic recycling, leading to functional resensitization of neurotrophic signaling." Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Non-tracable Author Statement (NAS): | This annotation required some inference over additional information/literature, and is not solely based on observations made in the annotated paper by its original authors. |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4804 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |