| Annotated protein: | Nck-associated protein 1 (NAP 1) (Brain protein H19) (MH19) (Membrane-associated protein HEM-2) (p125Nap1). Gene symbol: NCKAP1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P28660 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NCKAP1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | neocortex |
| Annotated paper: | De Rubeis S, et al. "CYFIP1 coordinates mRNA translation and cytoskeleton remodeling to ensure proper dendritic spine formation" Neuron. 2013 Sep 18;79(6):1169-82 PMID:24050404 |
| Figure(s): | Fig. 1B; 1C; 2A |
| Annotation description: | NCKAP1 is detected by Western blot after co-IP with CYFIP1 from synaptosome preparations from cortical mouse brain. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Alexandros Kanellopoulos (ORCID:0000-0002-2094-7491) Vittoria Mariano (ORCID:0000-0002-9848-0262) Achsel Tilmann (ORCID:0000-0002-1190-4481) Claudia Bagni (ORCID:0000-0002-4419-210X) |
| Lab: | Department of Fundamental Neurosciences, University of Lausanne, CH-1006 Lausanne, Switzerland; Dept Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy |
| Additional literature: | Supplemental Fig. 3: NCKAP1 (Nap1) deficiency (assessed by E15 electroporation of Nap1 shRNA) affects dendritic spine density in mouse cortical neurons (C57Bl6 mice) @ PMID:17481396 |
| SynGO annotation ID: | 480 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |