| Annotated protein: | Vang-like protein 2 (Van Gogh-like protein 2). Gene symbol: VANGL2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P84889 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VANGL2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Nagaoka T, et al. "PDZ interaction of Vangl2 links PSD-95 and Prickle2 but plays only a limited role in the synaptic localisation of Vangl2" Sci Rep. 2015 Aug 10;5:12916 PMID:26257100 |
| Figure(s): | Figure 2-4 |
| Annotation description: | Figure 2: immunofluorescence confocal microscopy showed PSD95 (DLG4) co-localized with Vangl2 at synaptic puncta in rat hippocampal neurons that were transfected with GFP-Vangl2. Co-localization was significantly reduced when using GFP-Vangl2-ΔETSV (disrupted PDZ-binding motif). Figure 3,4: Vangl2 can be localized to the postsynaptic density by both PDZ interaction-dependent and -independent mechanisms. Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | characterization of PSD95 and Vangl2 interaction. Also shows the effect of the PDZ-binding motif of Vangl2 to synaptic localization. @ PMID:23567299 confocal imaging shows co-localization with synaptic markers, but resolution is insufficient to discriminate subcellular localization as pre- or post-synaptic @ PMID:28057866 |
| SynGO annotation ID: | 4795 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |