| Annotated protein: | Prickle-like protein 1. Gene symbol: PRICKLE1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q3U5C7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PRICKLE1 |
| Ontology domain: | Biological Process |
| SynGO term: | maintenance of postsynaptic density structure (GO:0099562) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ban Y, et al. "Prickle promotes the formation and maintenance of glutamatergic synapses by stabilizing the intercellular planar cell polarity complex" Sci Adv. 2021 Oct 8;7(41):eabh2974 PMID:34613779 |
| Figure(s): | Figure 1, S2, 2 |
| Annotation description: | Prickle1 (PK1) and Prickle2 (PK2) are involved in synapse formation and maintenance. Figure 1, S2: "To address whether PK1 and PK2 are also engaged in regulating the number of glutamatergic synapses in adulthood, we injected sgRNA viruses into the same brain regions of the Cre-dependent Cas9 mice at the age of 8 to 9 weeks. At 12 weeks, we found PK1 deletion led to an average of 41.5% reduction, and PK2 deletion resulted in an average of 57.5% reduction. Thus, PK1 and PK2 are critical not only for the assembly of glutamatergic synapses but also for their maintenance in adulthood. To examine whether PK1 and PK2 are functionally overlapping, we performed double knockout by injection of both the PK1 sgRNA virus and the PK2 sgRNA virus to the dorsal HPC and mPFC of the Cre-dependent Cas9 knock-in mice. Double knockout caused an average reduction of 72.5 and 75% during development and adulthood, respectively." These findings were confirmed using conditional knockouts in Figure 2. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Super resolution Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4794 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |