| Annotated protein: | Prickle-like protein 2. Gene symbol: PRICKLE2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q80Y24 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PRICKLE2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic density assembly (GO:0099151) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ban Y, et al. "Prickle promotes the formation and maintenance of glutamatergic synapses by stabilizing the intercellular planar cell polarity complex" Sci Adv. 2021 Oct 8;7(41):eabh2974 PMID:34613779 |
| Figure(s): | Figure 1, S2, 2, 5, 6 |
| Annotation description: | Prickle1 (PK1) and Prickle2 (PK2) are involved in synapse formation and maintenance. Figure 1, S2: Authors used the CRISPR-Cas9 system to knockout Prickle1/Prickle2 after axon development was finished, to avoid secondary and indirect effects on synapse formation, and observed strongly reduced synapse formation. "We then quantified the number of glutamatergic synapses using immunostaining with presynaptic (Bassoon) and postsynaptic (PSD-95) markers and observed an average of 58.5% reduction in the number of synapses in PK1 knockout and an average of 43.5% decrease in PK2 knockout. These results suggest that both PK1 and PK2 are required for synapse formation during development." These findings were confirmed using conditional knockouts in Figure 2. Disruption of Prickle2 interactions with Vangl2 and the intercellular complex Celsr3/Fzd3-Celsr3 appear causal to impaired regulation of synapse assembly (Figure 5, 6) that was observed earlier in Prickle2 knockouts (Figure 1, 2). |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Super resolution IP + WB/MSMS Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4792 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |