| Annotated protein: | Prickle-like protein 2. Gene symbol: PRICKLE2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q80Y24 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PRICKLE2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic density assembly (GO:0099151) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Sowers LP, et al. "Disruption of the non-canonical Wnt gene PRICKLE2 leads to autism-like behaviors with evidence for hippocampal synaptic dysfunction" Mol Psychiatry. 2013 Oct;18(10):1077-89 PMID:23711981 |
| Figure(s): | Figure 3, 4 |
| Annotation description: | Disrupting mouse Prickle2 reduced dendrite arborization, synapse number, and synapse size in hippocampal neurons. "We assessed PSD size and number using transmission electron microscopy (TEM) in the hippocampus and amygdala of Prickle2-/- mice and Prickle2+/+ controls (Fig. 3). We found that PSD size and synapse number were significantly reduced in the Prickle2-/- hippocampus in both the dentate gyrus (DG) and CA1 (Fig. 3 a-c). Interestingly, given that Prickle2-/- mice had normal amygdala-dependent behavior, both PSD size and synapse number were normal in the lateral amygdala of Prickle2-/- mice (Fig. 3d)." "we assessed hippocampal dendrite morphology using cultured primary hippocampal Prickle2+/+ and Prickle2-/- neurons by Sholl Analysis (Fig. 4 d, e). Prickle2-/- neurons showed a significant decrease in dendritic arborization evidenced by fewer intersections (Fig. 4 d, e). Overexpression of GFP-tagged wild-type human PRICKLE2 (hPk2-GFP) in Prickle2+/+ neurons greatly increased dendritic complexity, and rescued deficits present in Prickle2-/- neurons (Fig. 4 e)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4788 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |