| Annotated protein: | Ankyrin-3 (ANK-3) (Ankyrin-G). Gene symbol: ANK3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O70511-3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ANK3 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of modification of postsynaptic structure (GO:0099159) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Smith KR, et al. "Psychiatric risk factor ANK3/ankyrin-G nanodomains regulate the structure and function of glutamatergic synapses" Neuron. 2014 Oct 22;84(2):399-415 PMID:25374361 |
| Figure(s): | Figure 4, S4, 5, S5 |
| Annotation description: | Figure 4, S4: "Overexpression of the ANK3 (ankyrin-G) 190 kDa isoform, GFPAnkG190, caused a 24% increase in spine area compared with control, contributed by both an increase in spine length and head width and no effect on spine density (Figure S4C), suggesting that at this mature time point, ankyrin-G190 is primarily modulating the maintenance of existing spines." Figure 5, S5: 190 kDa Ankyrin-G modulates spine head and neck width, based on SIM Imaging. "We analyzed neurons over-expressing GFPAnkG190 and found that the presence of GFPAnkG190 anywhere in the spine causes enlargement of head and neck width in mushroom spines compared to spines without GFPAnkG190 or spines from neurons expressing mCherry alone. This effect was specific to mushroom-type spines and not thin spines, although the overexpression of GFPAnkG190 caused no changes in the abundance of mushroom spines" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Super resolution Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | "Usp9X Modulates Spine Morphology through Ankyrin-G Stabilization" @ PMID:31813652 |
| SynGO annotation ID: | 4785 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |