| Annotated protein: | Ankyrin-3 (ANK-3) (Ankyrin-G). Gene symbol: ANK3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O70511-3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ANK3 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Smith KR, et al. "Psychiatric risk factor ANK3/ankyrin-G nanodomains regulate the structure and function of glutamatergic synapses" Neuron. 2014 Oct 22;84(2):399-415 PMID:25374361 |
| Figure(s): | Figure 2, S2 |
| Annotation description: | Figure 2, S2: high resolution imaging shows ANK3 (ankyrin-G) is localized to postsynapses, at both spine head and neck, in primary cultures of rat cortical neurons. Quantification of overlap between ANK3~Bassoon is similar to ANK3~PSD95 (Fig. S2G). Authors observed; "To confirm presence of ankyrin-G at synapses, we immunostained for ankyrin-G and either the presynaptic marker bassoon (Figure 2C) or postsynaptic scaffold PSD95 (Figure 2D); both markers exhibited substantial colocalization with ankyrin-G (Figure S2D)" Figure 4: the 190kDa short form of ANK3 is localized to spines. "Quantification of the fluorescence intensity of both isoforms in spines revealed 60% less spine localization of GFPAnkG270 compared with GFPAnkG190 and a reduced spine:shaft ratio (***p < 0.0002; Figures 4E and S4B), suggesting that the 190 kDa isoform is localized at synaptic sites in cortical neurons" Figure 8: ANK3 (ankyrin-G) has greater association with both the cell membrane and cytoskeleton following a learning task in mice. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) Over-expression |
| Evidence tracking, Experiment Assay: | Super resolution Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4784 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |