| Annotated protein: | Voltage-dependent calcium channel subunit alpha-2/delta-2 (Protein ducky) (Voltage-gated calcium channel subunit alpha-2/delta-2) [Cleaved into: Voltage-dependent calcium channel subunit alpha-2-2; Voltage-dependent calcium channel subunit delta-2]. Gene symbol: CACNA2D2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6PHS9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNA2D2 |
| Ontology domain: | Cellular Component |
| SynGO term: | anchored component of presynaptic active zone membrane (GO:0099029) |
| Synapse type(s): | hippocampus, GABAergic striatum, GABAergic |
| Annotated paper: | Geisler S, et al. "Presynaptic alpha(2)delta-2 Calcium Channel Subunits Regulate Postsynaptic GABA(A) Receptor Abundance and Axonal Wiring" J Neurosci. 2019 Apr 3;39(14):2581-2605 PMID:30683685 |
| Figure(s): | Figure 1-4, 6, 8 |
| Annotation description: | Figure 1,2,8: presynaptic overexpression of CACNA2D2 (α2δ-2 Calcium Channel) was enriched at synaptic puncta Figure 3,4: presynaptic overexpression of CACNA2D2 lead to increased postsynaptic GABAAR recruitment in cultured hippocampal and medium spiny neurons Figure 6: "live-cell labeling of MSNs cotransfected with HA-tagged CACNA2D subunits and soluble eGFP revealed that all CACNA2D isoforms are expressed on the surface of presynaptic axonal varicosities and thus can in theory contribute to synaptic functions in GABAergic neurons" Davies A, et al. 2010 (PMID:20080692, Fig. 4G) showed CACNA2D subunits are most likely linked to the plasma membrane via GPI anchor, hence we selected "anchored component of ... membrane" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Provides evidence that alpha_2 delta-1 is GPI anchored in the plasma membrane of cells. alpha_2 delta-1 is expressed on the plasma membrane of DRGs in culture (Fig. 4E, immunofluorescence) and incubation with phosphatidylinositol-phospholipase C (PI-PLC) causes it to be cleaved from the surface of cells. Alpha_2 delta-1 from hippocampus also partitions into cholesterol-rich detergent-resistant membrane (DRM) fractions - termed 'lipid rafts'. @ PMID:20080692 |
| SynGO annotation ID: | 4777 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |