| Annotated protein: | Neurexin III. Gene symbol: NRXN3. Taxonomy: Mus musculus (Mouse). Uniprot ID: E9Q2X2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NRXN3 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic active zone membrane (GO:0099059) |
| Synapse type(s): | hippocampus, GABAergic |
| Annotated paper: | Hauser D, et al. "Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition" Neuron. 2022 Jul 6;110(13):2094-2109.e10 PMID:35550065 |
| Figure(s): | Figure 3 |
| Annotation description: | "In stratum lacunosum moleculare (SLM) of CA1, an area rich in GABAergic synapses formed by SST interneurons (Figures 3A and S2K), NRXN3 HA-tagged-protein concentrates in structures containing SST and vesicular inhibitory amino acid transporter VIAAT (Figure 3B) and is apposed to the GABAergic postsynaptic marker Gephyrin (Figure 3C). By contrast, no apposition was observed for the glutamatergic postsynaptic marker PSD95 (Figure 3C). Pre-embedding immunoelectron microscopy in CA1 SLM showed significant labeling at symmetric synapses on dendritic shafts, but not asymmetric synapses on spines (Figures 3D, 3E, and S3A). The vertical distribution from the midline of the synaptic cleft to the center of metal particles peaked in a presynaptic 0-10-nm bin, with the mean distance of 13.4 ± 1.7 nm presynaptic from the midline of the synaptic cleft (Figure 3F). Considering the size of antibodies and gold particles, this biased distribution suggests an exclusive presynaptic localization of NRXN3 AS5HA. ... Thus, the selective tagging of the endogenous NRXN3 AS5 proteoform uncovered an unexpected localization at specific populations of GABAergic synapses in vivo." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Super resolution |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4772 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |