| Annotated protein: | Ras-related protein Rab-5A (EC 3.6.5.2) (Small GTP-binding protein rab5). Gene symbol: RAB5A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: M0RC99 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RAB5A |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic endosome (GO:0098830) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Ackermann F, et al. "Critical role for Piccolo in synaptic vesicle retrieval" Elife. 2019 May 10;8:e46629 PMID:31074746 |
| Figure(s): | Figure 4, 5 |
| Annotation description: | "we analyzed the recruitment of two endogenous endosome markers, Rab5 and EEA1, towards GFP-2x-FYVE puncta. Here, we analyzed the fraction of GFP-2x-FYVE/Rab5 double positive puncta as a measure for early endocytic vesicles, and the fraction of GFP-2x-FYVE/Rab5/EEA1 triple positive puncta as a measure for early endosomes (Figure 5A). For technical reasons this analysis was performed along axons without an additional co-staining for synapses. Of note, co-localization studies with Synaptophysin demonstrated that at least 65% of GFP-2x-FYVE positive puncta along axons were synaptic (Figure 4, figure supplement 2D and E), a concept previously reported at the Drosophila NMJ (Wucherpfennig et al., 2003). Therefore our analysis represents a mixture of PI3P-positive organelles in- and outside synapses." Here, GFP-2x-FYVE was used as a reporter for PI3P. Figure 5 shows fewer endosome proteins (EEA1, Rab5) were recruited towards PI3P-positive organelles along axons lacking Piccolo. Figure 5 figure supplement 1C/F showed PI3P/Rabex5/Rab5 triple-positive staining, suggesting Rab5 and Rabex5 were localized to endosomes at the presynapse. This is reduced in axons lacking Piccolo. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4769 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |