Annotated protein:	ArfGAP with GTPase domain, ankyrin repeat and PH domain 1. Gene symbol: AGAP1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A0G2K847
antibody wiki:	search antibodypedia...
SynGO gene info:	SynGO data @ AGAP1
Ontology domain:	Biological Process
SynGO term:	regulation of modification of postsynaptic structure (GO:0099159)
Annotated paper:	Arnold M, et al. "The Endosome Localized Arf-GAP AGAP1 Modulates Dendritic Spine Morphology Downstream of the Neurodevelopmental Disorder Factor Dysbindin" Front Cell Neurosci. 2016 Sep 22;10:218 PMID:27713690
Figure(s):	Figure 7
Annotation description:	Figure 7a,b: Pyramidal neurons that overexpressed WT AGAP1 had significantly lower dendritic spine density, with an average 40% reduction. On the other hand, siRNA-mediated AGAP1 knockdown caused a change in spine density that did not reach statistical significance. Figure 7c,d: Spine length and neck size were significantly increased by either up or down regulation of AGAP1. Both siRNA-mediated AGAP1 knockdown and AGAP1 overexpression altered the proportions of spine types from predominately mushroom spines to primarily immature spines (Figure 7D). The proportion of thin spines increased to ~60% after AGAP knockdown. "These data demonstrate that AGAP1 levels modulate both dendritic spine density and the proportion of morphological spine types in hippocampal pyramidal neurons."
Evidence tracking, Biological System:	Cultured neurons
Evidence tracking, Protein Targeting:	Over-expression RNAi / shRNA
Evidence tracking, Experiment Assay:	Confocal
Annotator(s):	Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:	Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:	4767
Dataset release (version):	20231201
View annotation as GO-CAM model: