Annotated protein:	Synaptic vesicular amine transporter (Monoamine transporter) (Solute carrier family 18 member 2) (Vesicular amine transporter 2) (VAT2). Gene symbol: SLC18A2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BRU6
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SynGO gene info:	SynGO data @ SLC18A2
Ontology domain:	Biological Process
SynGO term:	synaptic vesicle neurotransmitter loading (GO:0098700)
Synapse type(s):	striatum, dopaminergic
Annotated paper:	Wang YM, et al. "Knockout of the vesicular monoamine transporter 2 gene results in neonatal death and supersensitivity to cocaine and amphetamine" Neuron. 1997 Dec;19(6):1285-96 PMID:9427251
Figure(s):	5
Annotation description:	Uses the electrically evoked release of Dopamine in a wild type and VMAT deficient background.
Evidence tracking, Biological System:	Intact tissue
Evidence tracking, Protein Targeting:	Genetic transformation (eg; knockout, knockin, mutations)
Evidence tracking, Experiment Assay:	Electrophysiology (generic)
Annotator(s):	Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534)
Lab:	Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany
Additional literature:	Knockout of the VMAT2 gene and several levels of analysis. @ PMID:9427251 In this paper, bovine VMAT2 is overexpressed in COS cells. Crude cell-free extracts were prepared and incubated with [3H]noradrenaline. Uptake of NA was observed that was prevented by pre-incubation with the VMAT-specific inhibitor tetrabenazine (Fig. 1A). In addition, specific binding of tetrabenazine was observed. The pharmacolocical profile of both NA-uptake and tetrabenazine-binding corresponded to that previously described for vesicular monoamine transport (Fig. 2). @ PMID:8150075
SynGO annotation ID:	476
Dataset release (version):	20231201
View annotation as GO-CAM model: