Annotated protein:AP-2 complex subunit alpha. Gene symbol: AP2A1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZUY8
antibody wiki:
SynGO gene info:SynGO data @ AP2A1
Ontology domain:Cellular Component
SynGO term:synaptic vesicle (GO:0008021)
Annotated paper:Taoufiq Z, et al. "Hidden proteome of synaptic vesicles in the mammalian brain" Proc Natl Acad Sci U S A. 2020 Dec 29;117(52):33586-33596 PMID:33376223
Figure(s):Dataset S1
Annotation description:AP2A1 was detected in SV biochemical fractions, and enriched as compared to the P2 fraction. This protein was also detected in SV biochemical fractions in Takamori et al. 2006 (PMID: 17110340).
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:none of the above
Evidence tracking, Experiment Assay:Biochemical fractionation (generic)
Mass-spectrometry
Annotator(s):Frank Koopmans (ORCID:0000-0002-4973-5732)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
Additional literature:XP_218624 protein was identified in SV biochemical fractions @ PMID:17110340

immuno-EM showed with an antibody against AP2A1/2 showed localization to pre- and post-synapse in rat hippocampal neurons @ PMID:15322548
SynGO annotation ID:4758
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology