| Annotated protein: | AP-3 complex subunit mu-1 (AP-3 adaptor complex mu3A subunit) (Adaptor-related protein complex 3 subunit mu-1) (Mu-adaptin 3A) (Mu3A-adaptin). Gene symbol: AP3M1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9JKC8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ AP3M1 |
| Ontology domain: | Biological Process |
| SynGO term: | postsynaptic neurotransmitter receptor endocytosis (GO:0098884) |
| Annotated paper: | Steinmetz CC, et al. "Upregulation of mu3A Drives Homeostatic Plasticity by Rerouting AMPAR into the Recycling Endosomal Pathway" Cell Rep. 2016 Sep 6;16(10):2711-2722 PMID:27568566 |
| Figure(s): | Figure 3, 4, 6 |
| Annotation description: | Figure 3: immunohistochemistry localized AP3M1/2 (antibody is aspecific) to recycling endosomes (RE), early endosomes (EE) and lysosomes in both cell body and dendrites. Highest co-localization is with RE. Figure 4: shRNA knockdown of of AP3M1 (μ3A) specifically strongly reduced dendritic AP3M1/2 puncta (since the antibody is aspecific, remaining staining is probably AP3M2/μ3B). This knockdown also affected surface-expressed GluA2. Figure 6: AP3M1 (μ3A-FLAG) is necessary and sufficient to recruit GluA2 to recycling endosomes and enhances the ability of GRIP1 to recruit GluA2 to the dendritic surface |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4756 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |