| Annotated protein: | DOMON domain-containing protein FRRS1L (Ferric-chelate reductase 1-like protein). Gene symbol: FRRS1L. Taxonomy: Mus musculus (Mouse). Uniprot ID: B1AXV0 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ FRRS1L |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic ER membrane |
| Annotated paper: | Schwenk J, et al. "An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity" Neuron. 2019 Nov 20;104(4):680-692.e9 PMID:31604597 |
| Figure(s): | Figure 1, 2 |
| Annotation description: | Figure 1: biochemical fractions of ER-enriched and synaptic membranes show FRRS1L is present on both but enriched in the ER. Figure 2: Stepwise co-expression in cultured cells showed FRRS1L is able to form protein complexes together with GRIA1, GRIA2 and and various ER-expressed AMPAR auxilliary subunits. A minority (~30%) of surface-expressed FRRS1L in mouse dissociated hippocampal neuron cultures co-localizes with PSD95/DLG4 (W. Han et al. 2017, PMID:29276473), so it stands to reason that some (but not all) ER localized FRSS1L is localized at or very near postsynapses. Based on the known transmembrane topology of this protein, "integral component of ... membrane" term was selected. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | surface HA-FRRS1L (sHA-FRRS1L) in mouse dissociated hippocampal neuron cultures at DIV16 partially co-localizes (~50% of FRRS1L puncta) with surface GluA1 (sGluA1), but the majority of sHA-FRRS1L does not co-localize with an excitatory synapse marker, PSD-95 (~30% of FRRS1L puncta). @ PMID:29276473 immunoEM evidence for FRRS1L ER localization @ PMID:28675162 |
| SynGO annotation ID: | 4754 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |