| Annotated protein: | Calpain-2 catalytic subunit (EC 3.4.22.53) (80 kDa M-calpain subunit) (CALP80) (Calcium-activated neutral proteinase 2) (CANP 2) (Calpain M-type) (Calpain-2 large subunit) (Millimolar-calpain) (M-calpain). Gene symbol: CAPN2. Taxonomy: Mus musculus (Mouse). Uniprot ID: O08529 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CAPN2 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Liu GT, et al. "Endosomal phosphatidylinositol 3-phosphate controls synaptic vesicle cycling and neurotransmission" EMBO J. 2022 May 2;41(9):e109352 PMID:35318705 |
| Figure(s): | Figure 5, S3 |
| Annotation description: | "In PI(3)P-depleted neurons, p35-Cdk5 appeared to be proteolytically converted to the hyperactive p25-Cdk5 isoform, a reaction known to be catalyzed by the calcium-dependent protease Calpain (Shah & Lahiri, 2014). Consistent with this, we observed that specific pharmacological inhibition of Calpain by ALLN (Baudry & Bi, 2016) prevented p35-to-p25 conversion in CGNs (Fig 5E) and in hippocampal neurons (Fig EV3C). Importantly, ALLN completely occluded the inhibitory effect of PI(3)P depletion on SV endocytosis (Fig 5F). Occlusion of VPS34IN1-mediated inhibition of SV endocytosis was also observed, if Calpain activity was blocked by Calpeptin (Fig EV3B) or depletion of Calpain 2, but not Calpain 1 by shRNA-mediated knockdown (Figs 5G and H, and EV3D and E)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Antagonist / agonist |
| Evidence tracking, Experiment Assay: | pHluorin assay |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4744 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |