| Annotated protein: | Calpain-2 catalytic subunit (EC 3.4.22.53) (80 kDa M-calpain subunit) (CALP80) (Calcium-activated neutral proteinase 2) (CANP 2) (Calpain M-type) (Calpain-2 large subunit) (Millimolar-calpain) (M-calpain). Gene symbol: CAPN2. Taxonomy: Mus musculus (Mouse). Uniprot ID: O08529 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CAPN2 |
| Ontology domain: | Biological Process |
| SynGO term: | protein catabolic process at postsynapse (GO:0140249) |
| Annotated paper: | Zadran S, et al. "Brain-derived neurotrophic factor and epidermal growth factor activate neuronal m-calpain via mitogen-activated protein kinase-dependent phosphorylation" J Neurosci. 2010 Jan 20;30(3):1086-95 PMID:20089917 |
| Figure(s): | Figure 8, 9 |
| Annotation description: | Figure 8: BDNF-induced calpain activation was also monitored by Western blot analysis of spectrin breakdown product (SBDP), the truncated form of α-spectrin that is specifically generated by calpain-dependent cleavage (Saido et al., 1994). Neuronal cultures were incubated with BDNF or EGF for 60 min in the presence or absence of the MAPK pathway inhibitor PD98059. BDNF or EGF treatment of neurons increased SBDP accumulation, an effect that was blocked by pretreatment with PD98059. Figure 9: BDNF treatment increased serine phosphorylation of immunoprecipitated m-calpain (Fig. 9B). However, the low levels of μ-calpain did not allow determining potential changes in μ-calpain phosphorylation. Treatment with PD98059 alone resulted in decreased levels of serine-phosphorylated m-calpain, suggesting that m-calpain was constitutively phosphorylated by MAP kinase. "In conclusion, our results demonstrate that m-calpain is rapidly activated in dendrites and dendritic spines by BDNF as a result of MAP kinase-mediated phosphorylation and participates in cytoskeletal reorganization" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antagonist / agonist |
| Evidence tracking, Experiment Assay: | FRET Western blot |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | In cultured cortical neurons, knockdown of calpain-2, but not calpain-1, by small interfering RNA completely suppressed the effect of BDNF on mTOR activation. @ PMID:23467348 |
| SynGO annotation ID: | 4743 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |