| Annotated protein: | Calpain-2 catalytic subunit (EC 3.4.22.53) (80 kDa M-calpain subunit) (CALP80) (Calcium-activated neutral proteinase 2) (CANP 2) (Calpain M-type) (Calpain-2 large subunit) (Millimolar-calpain) (M-calpain). Gene symbol: CAPN2. Taxonomy: Mus musculus (Mouse). Uniprot ID: O08529 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CAPN2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Zadran S, et al. "Brain-derived neurotrophic factor and epidermal growth factor activate neuronal m-calpain via mitogen-activated protein kinase-dependent phosphorylation" J Neurosci. 2010 Jan 20;30(3):1086-95 PMID:20089917 |
| Figure(s): | Figure 3 |
| Annotation description: | Figure 3a: "Hippocampal neurons were preloaded with the FRET substrate and treated with BDNF (50 ng/ml) for 10 min or EGF (20 ng/ml) for 2 min. They were then fixed, stained with phalloidin-Alexa Fluor594, and processed for confocal microscopy. Decrease in FRET signal (increased fluorescence) was observed in dendrites as well as in dendritic spine-like structures labeled with phalloidin-Alexa Fluor594 (arrows)" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | FRET |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | calcium- and time-dependent m-calpain is released from synaptosomes in an activated form or in a form capable of activation, and this process is not a result of a violation of the integrity of synaptosomes @ PMID:33688783 same authors applied the same FRET assay. Figure 1B shows clear postsynaptic puncta for calpain 2 @ PMID:19995977 |
| SynGO annotation ID: | 4742 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |