| Annotated protein: | Calpain-1 catalytic subunit (EC 3.4.22.52) (Calcium-activated neutral proteinase 1) (CANP 1) (Calpain mu-type) (Calpain-1 large subunit) (Micromolar-calpain) (muCANP). Gene symbol: CAPN1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P97571 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CAPN1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Vanderklish PW, et al. "Marking synaptic activity in dendritic spines with a calpain substrate exhibiting fluorescence resonance energy transfer" Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2253-8 PMID:10688895 |
| Figure(s): | Figure 1, 4, 5 |
| Annotation description: | Figure 1: A calpain-sensitive marker, pYSCS, was designed with an intervening sequence from the μ-calpain (CAPN1, calpain 1) cleavage site in α-spectrin. A PDZ cognate sequence that binds to PSD95 was added at the 3' end to facilitate retention of the expressed protein at postsynaptic sites. Figure 4c: Expression of YSCS in dissociated hippocampal neurons showed YSCS is concentrated in spine heads. Figure 5: "The utility of pYSCS as a reporter of glutamate receptor-induced calpain activation was initially examined in real-time imaging experiments in cultures of dissociated hippocampal neurons. Images collected before and 3 to 5 min after exposure of neurons to glutamate or NMDA revealed a substantial decrease in the FRET ratio in dendritic spines (Fig. 5 A-F)" |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | FRET |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | confocal co-localization of calpain with Dlg at fruitfly PSD @ PMID:30705102 |
| SynGO annotation ID: | 4737 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |