| Annotated protein: | Phosphatidylinositol 3-kinase catalytic subunit type 3 (PI3-kinase type 3) (PI3K type 3) (PtdIns-3-kinase type 3) (EC 2.7.1.137) (Phosphoinositide-3-kinase class 3). Gene symbol: PIK3C3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6PF93 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PIK3C3 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Liu GT, et al. "Endosomal phosphatidylinositol 3-phosphate controls synaptic vesicle cycling and neurotransmission" EMBO J. 2022 May 2;41(9):e109352 PMID:35318705 |
| Figure(s): | Figure 2-4 |
| Annotation description: | Figure 2-4: monitoring SV endocytosis using Synaptophysin-pHluorin showed pharmacological inhibition of endosomal PI(3)P synthesis in hippocampal neurons caused impaired SV endocytosis in (stimulated) synapses. Authors conclude; "These data show that PI(3)P depletion perturbs SV endocytosis and reduces presynaptic release probability." As a control, selective inhibition of VPS34 by the well-established specific small molecule inhibitor VPS34IN1 (Bago et al, 2014, PMID:25177796; Ketel et al, 2016, PMID:26760201) effectively reduced somatic and synaptic PI(3)P to nearly undetectable levels (Figure S1). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Electron Microscopy pHluorin assay |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4736 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |