| Annotated protein: | Phosphatidylinositol 3-kinase catalytic subunit type 3 (PI3-kinase type 3) (PI3K type 3) (PtdIns-3-kinase type 3) (EC 2.7.1.137) (Phosphoinositide-3-kinase class 3). Gene symbol: PIK3C3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q6PF93 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PIK3C3 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Wang L, et al. "Pik3c3 deletion in pyramidal neurons results in loss of synapses, extensive gliosis and progressive neurodegeneration" Neuroscience. 2011 Jan 13;172:427-42 PMID:20955765 |
| Figure(s): | Figure 1b |
| Annotation description: | The lipid kinase PIK3C3 (VPS34) utilizes phosphatidylinositol as a substrate, producing the single lipid product phosphatidylinositol-3-phosphate (PI3P) (Wurmser et al., 1999, PMID:10092582). Figure 1b: Confocal images showed partial co-localization of a PI3P (mVenus-2xFYVE fusion protein containing a Venus tagged tandem FYVE domains that binds PI3P with high specificity) with PSD95 in DIV13 cultured hippocampal neurons. "To visualize the cellular distribution of its product PI3P in pyramidal neuron, we co-transfected hippocampal neurons with a construct containing a Venus (green) tagged tandem FYVE domains that binds PI3P with high specificity (Petiot et al., 2003) and mCherry (red) at 13 days in vitro (DIV). As shown in Figure 1A, Venus-2xFYVE signals were abundantly detected in the soma and dendrites (the picture was highly over-exposed intentionally to reveal the PI3P distribution in high order branches) with punctae-like fluorescence signals observed in higher order branches (Figure 1A). In the axon, very faint dotted-like fluorescence signals were detected (insert in Figure 1A). Immunostaining using antibody against postsynaptic marker PSD95 revealed that PI3P is enriched at the postsynaptic sites (Figure 1B). Venus-2xFYVE is not detectable in distal axon termini when moderately expressed, whereas high-level expression of venus-2xFYVE was toxic to the cultured neurons. Thus, we could not examine whether PI3P is present at the pre-synaptic sites." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4733 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |