| Annotated protein: | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 (EC 3.1.4.11) (PLC-154) (Phosphoinositide phospholipase C-beta-1) (Phospholipase C-beta-1) (PLC-beta-1). Gene symbol: PLCB1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9Z1B3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PLCB1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic cytosol (GO:0099524) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Fukaya M, et al. "Predominant expression of phospholipase Cbeta1 in telencephalic principal neurons and cerebellar interneurons, and its close association with related signaling molecules in somatodendritic neuronal elements" Eur J Neurosci. 2008 Nov;28(9):1744-59 PMID:18973591 |
| Figure(s): | Figure 3, 4 |
| Annotation description: | This study provides extensive characterization of PLC beta1 localization in brain, using immuno-electron microscopy (HRP labeling and immunogold pre- and postembedding). In CA1 pyramidal cells, PLC beta1 was predominantly detected in somatodendritic compartments, including dendritic spines (Figure 3, 4). No signal was observed in presynaptic terminals. Note that most signal is cytoplasmatic, although some membrane staining is observed. The latter might be due to activity dependent recruitment of PLC to the plasma membrane via its PH or C2 domains. In the cerebellum, PLC beta1 appears to be largely absent from the synapse (figure 8). A subcellular fractionation for PLC beta1 is also provided (Figure 3A). This result is in agreement with the EM data, but lacks controls. Extensive characterization of the antibody is provided, including knockout controls in western blot (Figure 1A, immunofluorescence (Figure 1B) and immuno-EM (Figure 3,4). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| SynGO annotation ID: | 473 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |