Annotated protein: | Epsin-2 (EPS-15-interacting protein 2). Gene symbol: EPN2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9Z1Z3 |
antibody wiki: | |
SynGO gene info: | SynGO data @ EPN2 |
Ontology domain: | Cellular Component |
SynGO term: | postsynaptic endocytic zone (GO:0098843) |
Synapse type(s): | hippocampus |
Annotated paper: | Catsburg LA, et al. "Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses" Elife. 2022 Jan 24;11:e74387 PMID:35072626 |
Figure(s): | Figures 4, 5 |
Annotation description: | Figure 4: Confocal imaging of hippocampal neuronal cultures with co-staining for Homer1c-mCherry and Epsn2-mCherry (EPN2, Epsin 2) show this protein is localized at the postsynapse, albeit less frequently than some other endocytic proteins. Figure 5: time-lapse experiments on neurons co-expressing Homer1c and fluorophore-tagged Epsn2 showed transient association with endocytic zones near the PSD, i.e. its average lifetime at perisynaptic sites was significantly lower compared to the average lifetime of CLCa. |
Evidence tracking, Biological System: | Cultured neurons |
Evidence tracking, Protein Targeting: | Over-expression |
Evidence tracking, Experiment Assay: | Confocal |
Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
SynGO annotation ID: | 4729 |
Dataset release (version): | 20231201 |
View annotation as GO-CAM model: |