Annotated protein:Epsin-2 (EPS-15-interacting protein 2). Gene symbol: EPN2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9Z1Z3
antibody wiki:
SynGO gene info:SynGO data @ EPN2
Ontology domain:Cellular Component
SynGO term:postsynaptic endocytic zone (GO:0098843)
Synapse type(s):hippocampus
Annotated paper:Catsburg LA, et al. "Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses" Elife. 2022 Jan 24;11:e74387 PMID:35072626
Figure(s):Figures 4, 5
Annotation description:Figure 4: Confocal imaging of hippocampal neuronal cultures with co-staining for Homer1c-mCherry and Epsn2-mCherry (EPN2, Epsin 2) show this protein is localized at the postsynapse, albeit less frequently than some other endocytic proteins.

Figure 5: time-lapse experiments on neurons co-expressing Homer1c and fluorophore-tagged Epsn2 showed transient association with endocytic zones near the PSD, i.e. its average lifetime at perisynaptic sites was significantly lower compared to the average lifetime of CLCa.
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Over-expression
Evidence tracking, Experiment Assay:Confocal
Annotator(s):Frank Koopmans (ORCID:0000-0002-4973-5732)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:4729
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology