Annotated protein:Dynamin-2 (EC 3.6.5.5). Gene symbol: DNM2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P39052
antibody wiki:
SynGO gene info:SynGO data @ DNM2
Ontology domain:Cellular Component
SynGO term:postsynaptic endocytic zone (GO:0098843)
Synapse type(s):hippocampus
Annotated paper:Catsburg LA, et al. "Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses" Elife. 2022 Jan 24;11:e74387 PMID:35072626
Figure(s):Figures 4-6
Annotation description:Figure 4: Confocal imaging of hippocampal neuronal cultures with co-staining for Homer1c-mCherry and Dyn2-GFP (DNM2) show this protein is localized at the postsynapse. Instead of GFP tagging, endogenous DNM2 was also tagged using CRISPR/Cas-9.

Figure 5: time-lapse experiments on neurons co-expressing Homer1c and fluorophore-tagged DNM2 showed high association with PSDs.

Figure 6: to dissect the spatial organization of endocytic proteins relative to the clathrin structure at
the endocytic zone (EZ), authors used two-color single-molecule localization microscopy (SMLM). Halo-CLCa co-transfected with GFP-tagged DNM2 showed that it was often distributed in smaller patches around and sometimes within the EZ marked by CLCa.
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Over-expression
Genetic transformation (eg; knockout, knockin, mutations)
Evidence tracking, Experiment Assay:Confocal
Super resolution
Annotator(s):Frank Koopmans (ORCID:0000-0002-4973-5732)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:4727
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology