| Annotated protein: | Epidermal growth factor receptor pathway substrate 15. Gene symbol: EPS15. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: E9PSY8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EPS15 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic endocytic zone (GO:0098843) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Catsburg LA, et al. "Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses" Elife. 2022 Jan 24;11:e74387 PMID:35072626 |
| Figure(s): | Figures 4-6 |
| Annotation description: | Figure 4: Confocal imaging of hippocampal neuronal cultures with co-staining for Homer1c-mCherry and EPS15-GFP show this protein is localized at the postsynapse. Instead of GFP tagging, endogenous EPS15 was also tagged using CRISPR/Cas-9. Figure 5: time-lapse experiments on neurons co-expressing Homer1c and fluorophore-tagged EPS15 showed high association with PSDs. Figure 6: to dissect the spatial organization of endocytic proteins relative to the clathrin structure at the endocytic zone (EZ), authors used two-color single-molecule localization microscopy (SMLM). Halo-CLCa co-transfected with GFP-tagged EPS15 showed that it was often distributed in smaller patches around and sometimes within the EZ marked by CLCa. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal Super resolution |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4725 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |