Annotated protein:	Intersectin-1 (EH domain and SH3 domain regulator of endocytosis 1). Gene symbol: ITSN1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9WVE9
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SynGO gene info:	SynGO data @ ITSN1
Ontology domain:	Cellular Component
SynGO term:	postsynaptic endocytic zone (GO:0098843)
Synapse type(s):	hippocampus
Annotated paper:	Catsburg LA, et al. "Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses" Elife. 2022 Jan 24;11:e74387 PMID:35072626
Figure(s):	Figures 4-6
Annotation description:	Figure 4: Confocal imaging of hippocampal neuronal cultures with co-staining for Homer1c-mCherry and GFP-Itsn1L (ITSN1) show this protein is localized at the postsynapse. Instead of GFP tagging, endogenous ITSN1 was also tagged using CRISPR/Cas-9. Figure 5: time-lapse experiments on neurons co-expressing Homer1c and fluorophore-tagged ITSN1 showed high association with PSDs. Figure 6: to dissect the spatial organization of endocytic proteins relative to the clathrin structure at the endocytic zone (EZ), authors used two-color single-molecule localization microscopy (SMLM). Halo-CLCa co-transfected with GFP-tagged ITSN1 showed that it was often distributed in smaller patches around and sometimes within the EZ marked by CLCa.
Evidence tracking, Biological System:	Cultured neurons
Evidence tracking, Protein Targeting:	Over-expression Genetic transformation (eg; knockout, knockin, mutations)
Evidence tracking, Experiment Assay:	Confocal Super resolution
Annotator(s):	Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:	Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:	4724
Dataset release (version):	20231201
View annotation as GO-CAM model: