| Annotated protein: | Metabotropic glutamate receptor 2 (mGluR2). Gene symbol: GRM2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P31421 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRM2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Bodzeta A, et al. "Subsynaptic mobility of presynaptic mGluR types is differentially regulated by intra- and extracellular interactions" Mol Biol Cell. 2022 Jul 1;33(8):ar66 PMID:35511883 |
| Figure(s): | Figure 1, S1, 2, S2 |
| Annotation description: | Figure 1,S1: Endogenous mGluR2 (GRM2) was tagged with a pH-sensitive variant of GFP using a CRISPR/Cas9-mediated knock-in approach. Super-resolution imaging of hippocampal neuronal cultures showed mGluR2 was localized both in axons and dendrites (Fig. S1A), and detected both in the axon shaft and within synaptic boutons (Fig. 1A). Co-staining with Bassoon (BSN) showed mGluR2 was localized near but not at the active zone (Fig. 1A). Figure 2,S2: Fluorescence recovery after photobleaching (FRAP) experiments showed surface-expressed mGluR2 was highly mobile and diffusely spread throughout the axon. A model for mGluRs 2 and 7 is proposed in Figure 6. Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Super resolution Fluorescence recovery after photobleaching (FRAP) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4720 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |