Annotated protein: | Calmodulin-1. Gene symbol: CALM1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P0DP26 |
antibody wiki: | |
SynGO gene info: | SynGO data @ CALM1 |
Ontology domain: | Biological Process |
SynGO term: | presynaptic endocytosis (GO:0140238) |
Synapse type(s): | Calyx of Held |
Annotated paper: | Jin YH, et al. "Protein Kinase C and Calmodulin Serve As Calcium Sensors for Calcium-Stimulated Endocytosis at Synapses" J Neurosci. 2019 Nov 27;39(48):9478-9490 PMID:31628181 |
Figure(s): | 6C-H, Figure 7C-K |
Annotation description: | Experiments use bioassays of endocytosis following stimulated exocytosis in calyx of held and hippocampal synapses in face of a Calmodulin 2 selective knockdown. The summed data supports a role for CaM2 in the efficient recycling of synaptic vesicles and in promoting access to the readily releasable pool. There are clear effects in face of Calmodulin 2 knockout despite evidencing residual levels of calmodulin accounted for by Calmodulin 1 and 3 expression (See Figure 6B and 7A and 7B). Based on the presynaptic expression of calmodulins from the Calm1 gene and the identical protein sequences generated from Calm1-3, this annotation infers that Calm1 has a similar presynaptic function as Calm2. |
Evidence tracking, Biological System: | Intact tissue Cultured neurons |
Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression |
Evidence tracking, Experiment Assay: | Electron Microscopy Whole-cell patch clamp Optical physiology |
Non-tracable Author Statement (NAS): | This annotation required some inference over additional information/literature, and is not solely based on observations made in the annotated paper by its original authors. |
Annotator(s): | Vincent O'Connor (ORCID:0000-0003-3185-5709) |
Lab: | Biological Sciences, Institute for Life Sciences, University of Southampton, Southampton, SO17 1BJ, United Kingdom |
SynGO annotation ID: | 4716 |
Dataset release (version): | 20231201 |
View annotation as GO-CAM model: |