| Annotated protein: | Myosin light chain kinase, smooth muscle (EC 2.7.11.18) (Telokin). Gene symbol: MYLK. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZFU9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MYLK |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synaptic vesicle endocytosis (GO:1900242) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Li L, et al. "Myosin light chain kinase facilitates endocytosis of synaptic vesicles at hippocampal boutons" J Neurochem. 2016 Jul;138(1):60-73 PMID:27062289 |
| Figure(s): | Figure 1, 4, 5 |
| Annotation description: | Both chemical inhibition of MLCK (MYLK) and down-regulation of MLCK with shRNAs impaired endocytosis following action potentials delivered at 5-40 Hz (Figures 1 and 4), whereas MLCK down-regulation did not change Ca2+channel current in the soma or vesicle exocytosis at boutons (Figure 5). Acute inhibition of myosin II similarly impaired endocytosis (Figure 6). Authors conclude that MLCK regulates endocytosis by functioning between Ca2+/calmodulin and Myosin. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antagonist / agonist RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4705 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |