| Annotated protein: | Pumilio RNA-binding family member 2. Gene symbol: PUM2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A0G2K8F9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PUM2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Vessey JP, et al. "Dendritic localization of the translational repressor Pumilio 2 and its contribution to dendritic stress granules" J Neurosci. 2006 Jun 14;26(24):6496-508 PMID:16775137 |
| Figure(s): | Figure 1c, 3b |
| Annotation description: | Figure 1c: Pum2 localizes within the dendritic compartment of mature hippocampal neuronal cultures. Figure 3b: "Pum2 is found in the dendritic shaft within close proximity of the base of dendritic spines. Immunostaining for Pum2 (red) and the postsynaptic marker PSD95 (green) demonstrates close apposition of PSD95 puncta and Pum2 particles" Figure 4b: "Dendritic Pum2 puncta contain mRNA. Immunostaining for Pum2 (green) was combined with FISH using an oligo-dT probe to detect mRNA. Pum2-containing puncta showed strong colocalization with mRNA(red). Some Pum2 puncta did not contain mRNA(arrows),whereas all mRNA contained Pum2(arrowheads)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | PUM and eIF4E studied in Dropsophila NMJ @ PMID:15541314 |
| SynGO annotation ID: | 4700 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |