| Annotated protein: | Neurogenic locus notch homolog protein 1 (Notch 1) (Motch A) (mT14) (p300) [Cleaved into: Notch 1 extracellular truncation (NEXT); Notch 1 intracellular domain (NICD)]. Gene symbol: NOTCH1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q01705 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NOTCH1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Alberi L, et al. "Activity-induced Notch signaling in neurons requires Arc/Arg3.1 and is essential for synaptic plasticity in hippocampal networks" Neuron. 2011 Feb 10;69(3):437-44 PMID:21315255 |
| Figure(s): | Figure 1C, 1F, S1B, 4A |
| Annotation description: | Authors used primary cultures from mouse hippocampal neurons to stain for PSD95 and Notch1 (Figure 1). There is co-localization between Notch1 and PSD95 (Figure 1C), while co-staining with synaptophysin is not so extensive (Figure S1B). Additionally, authors isolate postsynaptic densities biochemically and see increased Notch1 levels by western blot (Figure 1F). In Figure 4A, authors confirm the antibody specificity in Notch1 conditional knock out by observing a loss of staining in CA1 neurons at the hippocampus. "integral component of ... membrane" term was selected based on protein's known transmembrane topology. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Biochemical fractionation (generic) Western blot |
| Annotator(s): | Carlos Pascual-Caro (ORCID:0000-0002-2345-393X) Anjali Amrapali Vishwanath (ORCID:0000-0002-9888-0928) Jaime de Juan-Sanz (ORCID:0000-0002-1212-5623) |
| Lab: | ICM - Brain and Spine Institute, Hpital Piti Salptrire, 47 Boulevard de l'Hpital, 75013 Paris, France |
| SynGO annotation ID: | 4682 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |