| Annotated protein: | Exocyst complex component 7 (Exocyst complex component Exo70) (rExo70). Gene symbol: EXOC7. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O54922 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EXOC7 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synapse assembly (GO:0051963) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Lira M, et al. "The Exocyst Component Exo70 Modulates Dendrite Arbor Formation, Synapse Density, and Spine Maturation in Primary Hippocampal Neurons" Mol Neurobiol. 2019 Jul;56(7):4620-4638 PMID:30374940 |
| Figure(s): | Figure 2, 3, 7-9 |
| Annotation description: | Fig. 2: Overexpression of EXO70 increases dendritic branching (infection at DIV0, analysis at DIV15, MAP staining) Fig. 3: EXO70 shRNA rescues dendritic branching (infection at DIV0, analysis at DIV15, MAP staining): only 1 shRNA tested, no rescue with shRNA-resistant EXO70 Fig. 7: Overexpression increases, shRNA decreases number of Piccolo and Shank-positive synapses Fig. 8: Overexpression increases, shRNA decreases SV recycling measured with FM4-64 dye Fig. 9: Exo70 overexpression promotes spine maturation |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Ruud Toonen (ORCID:0000-0002-9900-4233) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4674 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |