| Annotated protein: | Adenylyl cyclase-associated protein 2 (CAP 2). Gene symbol: CAP2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P52481 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CAP2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Pelucchi S, et al. "Cyclase-associated protein 2 dimerization regulates cofilin in synaptic plasticity and Alzheimer's disease" Brain Commun. 2020 Jun 26;2(2):fcaa086 PMID:33094279 |
| Figure(s): | Figure 1, Figure 2A, Supplementary figure 2B |
| Annotation description: | Figure 1: WB showed the expression of cofilin, CAP2 and Tubulin in homogenate (HOMO) and TIF hippocampal samples of APP/PS1 mice at 6 months of age compared to wild type (WT) animals, as well as AD patients compared to matched controls. Figure 2A-B: confocal and STED of cultured rat hippocampal neurons fixed and stained for CAP2. Figure 2A: A linescan through the spine length shows that peaks of fluorescence for CAP2 and the presynaptic marker Bassoon do not overlap. Figure 2B: CAP2 fluorescence profile shows a partial overlap with the postsynaptic protein PSD-95 profile. Supplementary Fig. 2B: biochemical fractionation of rat hippocampi to isolate the PSD followed by WB analysis demonstrated that CAP2 is present in the synaptic fractions but not enriched in highly detergent-insoluble fractions PSD2. Synaptophysin and PSD95 were used as markers for the pre- and postsynapse. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Robin Clement Guus Smit Matthijs Verhage |
| ORCID: | 0000-0002-2286-1587 0000-0002-2514-0216 |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4672 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |