| Annotated protein: | Glutamate receptor ionotropic, delta-1 (GluD1) (GluR delta-1 subunit). Gene symbol: GRID1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q61627 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRID1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Dai J, et al. "GluD1 is a signal transduction device disguised as an ionotropic receptor" Nature. 2021 Jul;595(7866):261-265 PMID:34135511 |
| Figure(s): | Figure 1-2 |
| Annotation description: | Authors show that NRXN1(SS4+) and NRXN3(SS4+) regulate NMDARs and AMPARs via the cerebellin-dependent activation of GluDs that transduce distinct presynaptic neurexin signals into different postsynaptic receptor responses. The Neurexin alternative splicing site 4 (SS4) is critical to these signaling pathways. Figure 1-2. Patch-clamp recordings demonstrated that the GluD1 (GRID1) deletion (Western blot proof of KO in fig. 1B) increased both the frequency and amplitude of spontaneous miniature EPSCs (Fig. 1e), and enhanced the AMPA/NMDA ratio of evoked EPSCs (Fig. 1f, g). This enhancement was due to an increase in the amplitude of AMPAR EPSCs (Fig. 1h) and a decrease in the amplitude of NMDAR-EPSCs (Fig. 1i). Figure 1-2. Deleted GluD1 in the subiculum of young mice also increased the mEPSC frequency and amplitude (Fig. 1k), and enhanced the AMPA/NMDA ratio at CA1→subiculum synapses (Fig. 1l). Figure 2: Similar to GluD1 deletion, CBLN2 deletion increases AMPARs and decreases NMDARs. Both regulatory mechanisms operate at the same synapses. Double GluD1/CBLN2 deletion showed that CBLN2 and GluD1 operate in a unitary CBLN2-GluD1 signalling pathway that increases NMDAR EPSCs and decreases AMPAR EPSCs at individual synapses. Regarding GluD1, authors conclude "Together, these data indicate that the GluD1 deletion did not impair synapse formation, but altered the postsynaptic AMPAR and NMDAR composition." |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Robin Clement Guus Smit Matthijs Verhage |
| ORCID: | 0000-0002-2286-1587 0000-0002-2514-0216 |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | structures for GluD1 (GRID1), GluD2 (GRID2), Cbln1, Cbln1-GluD2 complex, β-NRX1-Cbln1-GluD2 complex @ PMID:27418511 |
| SynGO annotation ID: | 4670 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |