| Annotated protein: | Shroom family member 4. Gene symbol: SHROOM4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1LVL5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SHROOM4 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | hippocampus, glutamatergic hippocampus, GABAergic |
| Annotated paper: | Zapata J, et al. "Epilepsy and intellectual disability linked protein Shrm4 interaction with GABA(B)Rs shapes inhibitory neurotransmission" Nat Commun. 2017 Mar 6;8:14536 PMID:28262662 |
| Figure(s): | Fig. 1, Supplementary fig. 1, Table 1 |
| Annotation description: | Supplementary Fig. 1a The subcellular localization of Shrm4 was first investigated in cultured rat hippocampal neurons at 18 days in vitro (DIV) by immunostaining and found colocalization with presynaptic (Bassoon) and postsynaptic markers of excitatory (PSD-95) and inhibitory (GABAAb3) synapses (Supplementary Fig. 1a). Supplementary Fig. 1b; Supplementary Table 1 By using electron microscopy and post-embedding immunogold methods with an anti-Shrm4 antibody2, gold nanoparticles were identified in pre and post-synaptic areas and along dendrites (Supplementary Fig. 1b; Supplementary Table 1). Supplementary Fig. 1c Biochemical fractionation of adult rat brain hippocampi and cortices revealed enrichment of Shrm4 in the postsynaptic density (PSD) fraction further confirming its presence at synapses (Supplementary Fig. 1c). Fig. 1c; Supplementary Fig. 1e Moreover, direct stochastic optical reconstruction microscopy (dSTORM) of 14DIV hippocampal neurons revealed that Shrm4 and GABABRs co-cluster in neurons (Fig. 1c; Supplementary Fig. 1e). |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution Biochemical fractionation (generic) Electron Microscopy |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 4664 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |