| Annotated protein: | Teneurin-3 (Ten-3) (Protein Odd Oz/ten-m homolog 3) (Tenascin-M3) (Ten-m3) (Teneurin transmembrane protein 3). Gene symbol: TENM3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9WTS6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TENM3 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic active zone membrane (GO:0099059) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Zhang X, et al. "Teneurins assemble into presynaptic nanoclusters that promote synapse formation via postsynaptic non-teneurin ligands" Nat Commun. 2022 Apr 28;13(1):2297 PMID:35484136 |
| Figure(s): | Figure 2, S2 |
| Annotation description: | "Synaptic Tenm3 nanoclusters were separated by ~20-30 nm from both Bassoon and Homer1 macroclusters, suggesting that the Tenm3 nanoclusters are localized in the synaptic cleft (Fig. 2g, h, Supplementary Fig. 2c, f-h)." "The STORM results demonstrate that Tenm3 is an intrinsic component of nearly all synaptic junctions in the proximal CA1 region and distal subiculum. However, Tenm3 is not exclusively localized to synapses, but appears to be also present outside of synapses, similar to neurexins that also form presynaptic nanoclusters." "Our results show that Tenm3 protein is localized to the synaptic cleft, where it forms nanoclusters in presynaptic active zones. Moreover, our data document that pre- but not postsynaptic deletions of Tenm3 and Tenm4 produce robust impairments in synaptic connectivity. Thus Tenm3 and Tenm4 function in brain as presynaptic adhesion molecules that are essential for the formation and/or maintenance of at least a subset of excitatory synapses by binding to postsynaptic non-teneurin ligands since postsynaptic teneurins are not required." "integral component of ... membrane" term was selected based on protein's known transmembrane topology. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4658 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |