| Annotated protein: | Vezatin. Gene symbol: VEZT. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q3ZK22 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VEZT |
| Ontology domain: | Biological Process |
| SynGO term: | synapse maturation (GO:0060074) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Danglot L, et al. "Vezatin is essential for dendritic spine morphogenesis and functional synaptic maturation" J Neurosci. 2012 Jun 27;32(26):9007-22 PMID:22745500 |
| Figure(s): | Figure 3, 5 |
| Annotation description: | Figure 3. Vezatin knock-down induces less dendritic branching complexity in vitro. Figure 5. light microscopy shows a decrease in spines in a knockout mouse model that depleted Vezatin expression in forebrain. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Confocal Microscopy (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | in-situ hybridization showed Vezatin exhibited widespread gene expression with high levels in the hippocampus and medial habenular nucleus. Vezatin interacts with ARF6 in Y2H assay. In hippocampal neurons, Vezatin was localized at dendrites as well as cell bodies. Knockdown of endogenous Vezatin significantly reduced total dendritic length and arborization of cultured hippocampal neurons, while overexpression of Vezatin increased dendritic length @ PMID:20188128 Mechanistically, vezatin knockdown suppressed AMPAR-mediated synaptic events in epileptic mice and downregulated the surface expression of the AMPAR GluA1 subunit (GluA1). Interestingly, vezatin knockdown decreased the phosphorylation of GluA1 at serine 845 and reduced protein kinase A (PKA) phosphorylation; when PKA phosphorylation was suppressed by H-89 (a selective inhibitor of PKA phosphorylation) in vitro, the effects of vezatin knockdown on reducing the phosphorylation of GluA1 at serine 845 and the surface expression of GluA1 were blocked. @ PMID:34642320 vezatin has a selective role in the structural elaboration and postnatal maturation of murine neuromuscular synapses @ PMID:31411944 |
| SynGO annotation ID: | 4636 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |