| Annotated protein: | Roundabout homolog 2. Gene symbol: ROBO2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q7TPD3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ROBO2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Blockus H, et al. "Synaptogenic activity of the axon guidance molecule Robo2 underlies hippocampal circuit function" Cell Rep. 2021 Oct 19;37(3):109828 PMID:34686348 |
| Figure(s): | Figure 1, S1 |
| Annotation description: | Figure 1c. confocal microscopy colocalization with Homer1. "Subcellular localization of Robo2 in vitro. Ex utero electroporation of Robo2-pHluorin and Homer1 c-tdtomato in pyramidal neurons in vitro. Robo2-pHluorin colocalizes with the excitatory postsynaptic marker Homer1c at the majority of dendritic spines at 14 DIV" Figure S1. Supporting figure 1, confocal images of immunofluorescent staining for Robo2, Bassoon and PSD95 in CA1 stratum radiatum at P35. "High magnification of a putative synapse visualized by direct apposition of Bassoon and PSD95 shows strong enrichment of Robo2 with PSD95 suggesting postsynaptic localization" Figure 1d. westernblots show Robo2 localized to pre- and post-synaptic fractions (enriched in the latter). Annotated to postsynaptic density based on proximity to the presynapse in imaging data, visualization of plasma membrane-targeted form of ROBO2 (Fig. S1C-E) and interaction with neurexin. Based on the membrane topology of the protein, "integral component of ... membrane" ontology term was selected. |
| Evidence tracking, Biological System: | Cultured neurons Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4631 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |