| Annotated protein: | Kinesin heavy chain isoform 5A (Kinesin heavy chain neuron-specific 1) (Neuronal kinesin heavy chain) (NKHC). Gene symbol: KIF5A. Taxonomy: Mus musculus (Mouse). Uniprot ID: P33175 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KIF5A |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic cytosol (GO:0099524) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Zhao J, et al. "Specific depletion of the motor protein KIF5B leads to deficits in dendritic transport, synaptic plasticity and memory" Elife. 2020 Jan 21;9:e53456 PMID:31961321 |
| Figure(s): | Figure 1, S1 |
| Annotation description: | Figure 1B. western blot shows KIF5A,B,C were present in synaptic membrane fractions from mouse hippocampus. Figure S2. Confocal imaging of dissociated hippocampal neurons transfected with tdTomato and equal amounts of either KIF5A-GFP or KIF5B-GFP show puncta present in the heads of dendritic spines. Similar results were obtained from labeling with GFP and immunostaining with specific antibodies against either KIF5A or KIF5B. Further assays show that disruptions of KIF5A and KIF5B distinctly affects spine morphogenesis, plasticity and dendritic transport. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4630 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |