| Annotated protein: | Kinesin-1 heavy chain (Conventional kinesin heavy chain) (Ubiquitous kinesin heavy chain) (UKHC). Gene symbol: KIF5B. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q2PQA9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ KIF5B |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic cytosol (GO:0099524) |
| Synapse type(s): | hippocampus cerebral cortex |
| Annotated paper: | Twelvetrees AE, et al. "Delivery of GABAARs to synapses is mediated by HAP1-KIF5 and disrupted by mutant huntingtin" Neuron. 2010 Jan 14;65(1):53-65 PMID:20152113 |
| Figure(s): | Fig 2, 3, 4 |
| Annotation description: | Figure 2A, S2. Immunofluorescence and confocal microscopy with antibodies to the GABAAR g2 subunit and antibodies specific to KIF5B show that a proportion of KIF5B staining colocalized with GABAAR clusters in the neuronal somata and dendrites of cultured hippocampal and cortical neurons. Figure 2L,O, 3A-D. Pulldown assays with antibodies, GST fusion proteins, and mutated domains as controls, reveal HAP1 interacts with KIF5B. Further, mutated KIF5B effects GABAARs insertion into the surface membrane through the KIF5-HAP1 complex (Fig 3H, 4F). This supports the KIF5B-GABAAR colocalization observed earlier. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4628 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |