| Annotated protein: | Glutamate receptor ionotropic, kainate 3 (GluK3) (Glutamate receptor 7) (GluR-7) (GluR7). Gene symbol: GRIK3. Taxonomy: Mus musculus (Mouse). Uniprot ID: B1AS29 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRIK3 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Watanabe-Iida I, et al. "Determination of kainate receptor subunit ratios in mouse brain using novel chimeric protein standards" J Neurochem. 2016 Jan;136(2):295-305 PMID:26448475 |
| Figure(s): | Figure 6, table 1 |
| Annotation description: | Figure 1c. In situ hybridization in mouse brain shows GluK3 mRNA was moderately expressed in the cerebral cortex, hippocampus and cerebellum. GluK3 mRNA was detected in granule cells in the dentate gyrus, interneurons in the hippocampal Ammon's horn, and interneurons in the cerebellum (Fig. 1h,m and r). Figure 2. western blot (WB) characterization of the anti-GluK3 antibody in hippocampal lysate of wild type and GluK3-knockout mice. Figure 4, 5. GluK3 was detected by western blot in PSD biochemical fractions from mouse hippocampus and cerebellum. Quantitative analysis of these observations show strong enrichment in PSD as compared to the P2 fraction (fig. 6). Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4627 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |