| Annotated protein: | Protein lin-7 homolog B (Lin-7B) (Mammalian lin-seven protein 2) (MALS-2) (Vertebrate lin-7 homolog 2) (Veli-2). Gene symbol: LIN7B. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9Z252 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LIN7B |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density, intracellular component (GO:0099092) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Hu HT, et al. "Calcium influx and postsynaptic proteins coordinate the dendritic filopodium-spine transition" Dev Neurobiol. 2014 Oct;74(10):1011-29 PMID:24753440 |
| Figure(s): | Figure 6, 7 |
| Annotation description: | Figure 6b,c. Confocal imaging of cultured rat hippocampal SDC2-overexpressing neurons at DIV2 shows localization of CASK, LIN7B and surface-NR1 (GRIN1) at the tip of dendritic spines. Figure 7. Mutating the C2 motif of SDC2 leads to disruption of the SDC2-CASK-LIN7B-NMDAR complex, and consequently the localization of CASK, LIN7B and NR2B at the postsynaptic density was significantly reduced. Figure 8. Disruption of the SDC2-CASK and CASK-LIN7B interactions reduces NMDA-induced calcium influx and impairs SDC2-induced spine formation. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Figure 4 shows synaptic co-localization for LIN7B and SYP in rat primary cultured hippocampal neurons, but it cannot be ruled out that this is LIN7A/C based on the antibody a-specificity as also noted by authors "Using GST-Lin-7B expressed in E. coli as an antigen, a rabbit polyclonal antibody specific for Lin-7B was generated and affinity-purified on a column to which the antigen had been conjugated." "Since anti-Lin-7 was raised against full length human Lin-7B which contains the conserved regions among Lin-7A/MALS-1/Veli-1, -7B and -7C/MALS-3/Veli-3, this antibody is considered to recognize the three Lin-7 isoforms" (Figure 3) @ PMID:16979770 original characterization of vertebrate LIN-7 homologs Veli1-3 (LIN7A,B,C). Identification of cask/mint/lin7 complexes; PPIs evidence for LIN7A-C with Mint1 (APBA1) and CASK. LIN7A-C can also interact with DLG2 and DLG3. So this paper connects LIN7B with synaptic proteins @ PMID:9753324 |
| SynGO annotation ID: | 4624 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |