| Annotated protein: | Trafficking protein particle complex subunit 4 (Synbindin) (TRS23 homolog). Gene symbol: TRAPPC4. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9ES56 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TRAPPC4 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic active zone (GO:0048786) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Ethell IM, et al. "Synbindin, A novel syndecan-2-binding protein in neuronal dendritic spines" J Cell Biol. 2000 Oct 2;151(1):53-68 PMID:11018053 |
| Figure(s): | Figure 8, 10 |
| Annotation description: | "Figure 8. Double immunostaining of 30 DIV hippocampal neurons with antisynaptophysin (H) and antisynbindin (I) antibodies and their superimposed image (J)." > partial overlap can be observed "Immunogold labeling for synbindin was concentrated at asymmetric synapses (Fig. 10, A-D). Labeling was seen on both pre- and postsynaptic membranes, but the majority of the labeling was on the postsynaptic side in close association with postsynaptic membranes." "Immunogold labeling demonstrated that synbindin is also associated with membrane-bound compartments within neurons (Fig. 10, H-J). Consistent with the light microscopic findings (Fig. 7), immunogold labeling for synbindin was observed in the soma and dendritic shafts of cortical neurons. In these sites, labeling was associated predominantly with various cisterns and vesicles. Dense labeling was observed on the Golgi apparatus (Fig. 10H and Fig. I) and unidentified vesicles in the dendritic shaft (Fig. 10 J). Membrane compartments within dendritic spines were also labeled (Fig. 10 A, arrow). These observations demonstrate that synbindin is indeed associated with intracellular cisterns and vesicles, supporting the notion that synbindin is involved in vesicular transport and membrane trafficking in dendrites." "Thus, these immunoelectron microscopy results indicate that synbindin is localized in two distinct subcellular structures in neurons: (1) synapses (mainly postsynaptic membranes and PSD) and (2) intracellular membrane cisterns." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4621 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |