| Annotated protein: | Neurotrimin (GP65). Gene symbol: NTM. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q62718 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NTM |
| Ontology domain: | Cellular Component |
| SynGO term: | anchored component of presynaptic active zone membrane (GO:0099029) |
| Synapse type(s): | cerebellum Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Chen S, et al. "Neurotrimin expression during cerebellar development suggests roles in axon fasciculation and synaptogenesis" J Neurocytol. 2001 Nov;30(11):927-37 PMID:12373100 |
| Figure(s): | Figure 4, 5, 6 |
| Annotation description: | Figure 4a,b. immunogold labeling of neurotrimin at immature synaptic sites throughout synaptogenesis, increasing from P8-P14 and decreasing towards adulthood, where it is observed less in presynapses and most prominent at postsynaptic spines (Fig. 6a). "During granule cell migration and mossy fiber synaptogenesis, Ntm-immunoreactivity was most intense on granule cell surfaces (Fig. 5A and B) as well as between granule cell dendrites and mossy fiber terminals (Fig. 5A and C). With decreased immunoreactivity on granule cell somata and dendrites after P21, arrays of synaptic junctions appeared prominently around mossy fiber terminals as several synaptic contacts between mossy fibers and granule cell dendrites (Fig. 6F)." Annotated as "anchored component of ... membrane" based on GPI anchor. Source: "All members of this subfamily contain three Ig-like domains that share a high degree of homology and are anchored to the plasma membrane by a glycosylphosphatidylinositol moiety" (GPI anchor) (PMID:12373100). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | original characterization of neurotrimin @ PMID:7891157 |
| SynGO annotation ID: | 4615 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |