| Annotated protein: | Limbic system-associated membrane protein (LSAMP). Gene symbol: LSAMP. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q62813 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LSAMP |
| Ontology domain: | Cellular Component |
| SynGO term: | anchored component of presynaptic membrane (GO:0099026) |
| Annotated paper: | Zacco A, et al. "Isolation, biochemical characterization and ultrastructural analysis of the limbic system-associated membrane protein (LAMP), a protein expressed by neurons comprising functional neural circuits" J Neurosci. 1990 Jan;10(1):73-90 PMID:1688937 |
| Figure(s): | Figure 11, 12 |
| Annotation description: | Figures 11 and 12 show localization at pre- and post-synaptic membranes using immunogold labeling of adult and developing brain. Note that authors refer to limbic system-associated membrane protein as "LAMP" (current gene symbol is "LSAMP"). "lmmunocytochemical analysis at the ultra-structural level reveals that LAMP immunoreactivity is exhibited by neurons in a stereotyped pattern throughout limbic system areas. Glial cells are not immunoreactive. In the adult, LAMP-immunoreactive membrane patches are present exclusively postsynaptically on neuronal somata and dendrites. Myelinated and unmyelinated axons are not stained in any brain region examined. Analysis of LAMP expression in the developing CNS during synaptogenesis demonstrates that LAMP is located on growing axons and both pre- and postsynaptically at forming terminal complexes." Annotated as "anchored component of ... membrane" based on GPI anchor. Source: "All members of this subfamily contain three Ig-like domains that share a high degree of homology and are anchored to the plasma membrane by a glycosylphosphatidylinositol moiety" (GPI anchor) (PMID:12373100). |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | more EM evidence for synaptic localization, by same author (Pat Levitt) @ PMID:2922110 |
| SynGO annotation ID: | 4611 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |