Annotated protein:AP-2 complex subunit alpha-2 (100 kDa coated vesicle protein C) (Adaptor protein complex AP-2 subunit alpha-2) (Adaptor-related protein complex 2 subunit alpha-2) (Alpha-adaptin C) (Alpha2-adaptin) (Clathrin assembly protein complex 2 alpha-C large chain) (Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit). Gene symbol: AP2A2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P18484
antibody wiki:
SynGO gene info:SynGO data @ AP2A2
Ontology domain:Cellular Component
SynGO term:synapse (GO:0045202)
Annotated paper:Takei K, et al. "The synaptic vesicle cycle: a single vesicle budding step involving clathrin and dynamin" J Cell Biol. 1996 Jun;133(6):1237-50 PMID:8682861
Figure(s):Figure 2
Annotation description:This annotation is based on inference over multiple papers, as localization of AP2A2 to synapses and implication of the AP2 complex in synaptic endocytosis, for papers that we reviewed, use antibodies that are a-specific for AP2A1 and AP2A2.

However, additional mass-spectrometric evidence points to AP2A2 in subcellular enrichments of synaptic fractions for presynapse, postsynapse and synaptic vesicles (where enrichment is observed as compared to P2 fraction).

In figure 2, the authors prepare synaptosomes (nerve terminals) that they treat with GTPgammaS to trap endocytic vesicles. They then use immunogold with an antibody against AP-2 alpha adaptin to demonstrate the protein at endocytic sites. The antibody used, anti-alpha Adaptin antibody [AP6] (also available as Abcam #2730 / ab2730), targets both AP2A1 and AP2A2.
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Electron Microscopy
Non-tracable Author Statement (NAS):This annotation required some inference over additional information/literature, and is not solely based on observations made in the annotated paper by its original authors.
Annotator(s):Peter McPherson (ORCID:0000-0001-7806-5662)
Lab:Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada
Additional literature:AP2 protein complex structure from Cryo-EM (PDB:6OWO) shows a model with constituents Ap2a2, Ap2b1, Ap2m1, Ap2s1, Necap2 (specifically, uniprot:P17427 which is mouse AP2A2) @ PMID:31464684

presynaptic fraction, measured by LC-MS/MS.

mouse hippocampus: table S1, identifier GI:40254646 which corresponds to uniprot ID P17427 (mouse AP2A2), but note that detection was based on just 1 peptide.

rat striatum: table S2, identifier GI:51859448 which corresponds to uniprot ID Q66HM2 (rat AP2A2), but note that detection was based on just 1 peptide. @ PMID:19562802

Biochemical enrichment of PSD fraction from mouse hippocampi.

table S2, identifier GI:40254646 which corresponds to uniprot ID P17427 (mouse AP2A2), based on the detection of 4 peptides. @ PMID:17028301

identifies AP2A2 at in stringent biochemical purification of synaptic vesicles, measured by LC-MS/MS.

table S1, identifier P18484 (rat AP2A2) was ~3 fold enriched in SV compared to P2 fractions, based on 300+ peptide to spectrum matches (PSM) in each of the 3 replicates. @ PMID:33376223

identifies AP2A2 at in stringent biochemical purification of synaptic vesicles, measured by LC-MS/MS.

table S1, identifier NP_112270 corresponds to uniprot ID Q66HM2 (rat AP2A2), based on 29 peptides in 1D gel experiment and 15 peptides in 2D gel experiment. @ PMID:17110340

immunoisolated synaptic vesicles from rat brain, three distinct gel-based analyses of the synaptic vesicle proteome

table 1, identifier AAH58099 corresponds to uniprot ID Q6PEE6 (mouse AP2A2), based on all 3 SV purification protocols tested in this manuscript. @ PMID:17080482

characterizes various antibodies against AP2A1L (αAL), AP2A1S (αAS) and AP2A2 (αC) @ PMID:7593326

characterizes various antibodies against AP2A1L (αAL), AP2A1S (αAS) and AP2A2 (αC) @ PMID:34820873
SynGO annotation ID:4608
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology