| Annotated protein: | Protein smoothened. Gene symbol: SMO. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P97698 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SMO |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Annotated paper: | Petralia RS, et al. "Subcellular localization of Patched and Smoothened, the receptors for Sonic hedgehog signaling, in the hippocampal neuron" J Comp Neurol. 2011 Dec 15;519(18):3684-99 PMID:21618238 |
| Figure(s): | Figure 3, 4, 5, 8 |
| Annotation description: | Figure 3. Immunogold labeling for Smo in P2 hippocampus shows localization in the growth cones of newly generated migrating neurons. Figures 4. Immunogold labeling shows Smo at postsynapses in mature neurons in the CA1 and CA3 stratum radiatum of the adult hippocampus. "In addition to the plasma membrane, Smo was often associated with a variety of cytoplasmic organelles, including clathrin-coated vesicles (Fig. 3C) and different types of endosomes (Fig. 3E,H,J)." "In some synapses, Smo labeling was more intense in dendrites than in spines (Fig. 5C,H), whereas in other synapses, spine Smo was noticeable (Fig. 5D and inset). We also examined postembedding immunogold-labeled tissues. Consistent with the DAB labeling, Ptch immunogold particles were frequently found in spines (Fig. 5E,F), whereas Smo immunogold particles were present in some spines but were far more abundant in dendrites (Fig. 5G,I)." Beyond postsynaptic localization, Figure 8 suggests Smo may associate with synaptic spinules and autophagosomes in the CA3 mossy terminal area of the adult hippocampus. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4604 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |