| Annotated protein: | Patched (Patched 1). Gene symbol: PTCH1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q6UY90 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PTCH1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Annotated paper: | Petralia RS, et al. "Subcellular localization of Patched and Smoothened, the receptors for Sonic hedgehog signaling, in the hippocampal neuron" J Comp Neurol. 2011 Dec 15;519(18):3684-99 PMID:21618238 |
| Figure(s): | Figure 2, 3, 4, 5, 8 |
| Annotation description: | Figure 2E-F. Immunofluorescence labeling of Ptch in mature hippocampal neurons (20 div) shows localization in dendrites as well as puncta in spines, which overlap with presynaptic marker bassoon. Figure 3. Immunogold labeling for Ptch in P2 hippocampus shows localization in the growth cones of newly generated migrating neurons. Figures 4 and 5. Immunogold labeling shows Ptch at postsynapses of mature neurons in the CA1 and CA3 stratum radiatum of the adult hippocampus (Fig 4) and specifically enriched in spine heads (Fig. 5A,B). Figure 6A,C,E,G. Postsynaptic distribution of Ptch in the dentate gyrus is enriched in spines and spine heads. Beyond postsynaptic localization, Figure 8 suggests Ptch may associate with synaptic spinules and autophagosomes in the CA3 mossy terminal area of the adult hippocampus. based on the protein's transmembrane topology, "integral component of ..." was selected |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 4603 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |